High-throughput single cell RNA-sequencing of 120,196 CD34+Lin- HSPCs from 21 donors reveals marked expansion of megakaryocyte progenitors (MkP) in myelofibrosis.
note: The objects was built based on SingCellaR beta version. The figures are reproduced using SingCellaR v1.2.0
load the library and object
library(SingCellaR)
## Warning: replacing previous import 'ComplexHeatmap::pheatmap' by
## 'pheatmap::pheatmap' when loading 'SingCellaR'
library(ggplot2)
load(file = "~/Downloads/Psaila_Wang_MC/All_merged_combat_v1.rdata")
2B: Expression of lineage signature gene sets were superimposed on the UMAP (grey = uncommitted or expression of >1 lineage gene set, See also Suppl. Table 5).Brown - MF, Blue - HD.

2C: Cells in UMAP plot coloured according to donor type (healthy donors – blue; myelofibrosis – red). The gmt file can be found in this github repository.
